Black Stars captain Andre Ayew, his brother Jordan Ayew and Vice captain Thomas Partey will be available for selection after missing the game against South Africa due to issues related to the COVID-19 restrictions.
The three England based players joined the team in training on Friday at the Accra Sports Stadium ahead of the São Tomé and Principe game on Sunday.
Ghana will host bottom-placed Sao Tome and Principe in their last match of the series in Accra on Sunday after securing a place at next year’s Africa Cup of Nations in Cameroon, drawing 1-1 with South Africa.
The game against São Tomé and Principe will be played at 4pm on Sunday at the Accra Sports stadium.